Journal: bioRxiv

Article Title: Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs

doi: 10.1101/2022.06.19.496705

Figure Lengend Snippet: Cell surface fluorescence probing the extracellular part of PDGFRβ. A) Fluorescence microscopy images. Nuclei are in blue, FLAG-tag is in magenta, and PDGFRβ is in green. Scale bar is 5 μm.

Article Snippet: On ice, the cells were washed in ice cold PBS and incubated with a primary antibody targeting the extracellular part of PDGFRβ, 5 μg/ml goat-anti-PDGFRβ (AF385, RnD Systems) in PBS for 1 hour.

Techniques: Fluorescence, Microscopy, FLAG-tag

Journal: Neurologia medico-chirurgica

Article Title: Platelet-derived Growth Factor Activates Pericytes in the Microvessels of Chronic Subdural Hematoma Outer Membranes.

doi: 10.2176/jns-nmc.2023-0079

Figure Lengend Snippet: Fig. 1 (A) The outer membranes of chronic subdural hema- tomas (CSDHs) from 8 patients were homogenized in sample buffer and used for western blot analysis with anti-NG2, anti- N-cadherin, anti-VE-cadherin, anti-Tie-2, anti-endothelial ni- tric oxide synthase (eNOS), and anti-β actin antibodies. Nota- bly, all these molecules could be detected in the outer membranes of CSDHs. Positive controls are shown in the right 4 lanes, indicating that these molecules were correctly detect- ed. Liver lysate, rat liver whole cell lysate; NIH/3T3, embryo fi- broblast cell lysate; Endothelial cell, endothelial cell lysate; A375 cell lysate, malignant melanoma cell lysate. (B) Western blot analysis of the same samples was performed with anti-phosphorylated platelet-derived growth factor receptor-β at Tyr751 (p-PDGFR β at Tyr751), anti-PDGFR-β, and anti-β actin antibodies. p-PDGFR β at Tyr751, the activated form of PDGFR-β, was detected. Negative CNT, rat brain whole ly- sate, was used as a negative control; positive CNT, rat spleen whole lysate, was used as a positive control.

Article Snippet: The membranes were incubated with primary polyclonal antibodies against β-actin (SigmaAldrich, St. Louis, MO), PDGFR-β phosphorylated at Tyr751 (p-PDGFR-β at Tyr751, Cell Signaling Technology, Danvers, MA), NG2 (Gene Tex, Irvine, CA), N-cadherin (Cell Signaling Technology), VE-cadherin (Cell Signaling Technology), and Tie-2 (R&D Systems, Inc. Minneapolis, MN) and primary monoclonal antibodies against endothelial nitric oxide synthase (eNOS, BD Transduction Lab., Franklin Lakes, NJ) at a dilution of 1:750 overnight at 4°C.

Techniques: Western Blot, Derivative Assay, Negative Control, Positive Control